A Comparative Analysis of Growth and Polyhydroxybutyrate
production in Selected Strains of Bacteria
|
International Journal of Biotech Trends and Technology (IJBTT) | |
© 2015 by IJBTT Journal | ||
Volume-13 |
||
Year of Publication : 2015 | ||
Authors : Reji. S R, Nair G M, Gangaprasad A, Potty V P |
Citation
Reji. S R, Nair G M, Gangaprasad A, Potty V P "A Comparative Analysis of Growth and Polyhydroxybutyrate production in Selected Strains of Bacteria", International Journal of Biotech Trends and Technology (IJBTT),V13:8-13 November-December 2015. Published by Seventh Sense Research Group.
Abstract
Polyhydroxybutyrate (PHB) is a class of polyester accumulated intracellularly by many gram positive and gram negative bacteria as carbon and energy reserve granule. The potential of PHB as biodegradable plastic has been long recognized but several factors like time of extraction, method of extraction, carbon sources etc. hampered their commercialization for a wide range of application. So this study was aimed for isolating potential PHB accumulating strains and optimizing time of extraction of PHB from the strains. Time course study of growth and PHB production of bacteria revealed that optimum PHB production was associated with the stationary phase of its growth.
References
[1] H.Braldn, A. Gross, R. W. Lenz, R. W. Fuller, “ Plastic from
bacteria and for bacteria (polyhydroxyalkonates) as natural bio
compactable and biodegradable polyesters,” Adv. Biochem. Eng.
Biotechnol. 41, 77-93, 1990.
[2] R. M. Atlas, R. Bartha,“Microbial ecology: Fundamentals and
applications (ed. Atlas R M and BarthaR ). The
Benjamine/Cummings publishing company Inc,” New York.
1993.
[3] P. R. Patwardhan, A. K. Srivastava,“Model-based fed-batch
cultivation of R.eutrophafor enhanced biopolymer
production,”.Biochem. Eng. J. 20, 21-28, 2004.
[4] A. Khare, S. Deshmukh, “Studies toward producing ecofriendly
plastics,” Journal of Plastic Film and Sheeting. 22, 193-
211, 2006.
[5] C. Nawrath, Y. Poirier, C. Somerville,“Plant polymers for
biodegradable plastics:cellulose, starch and
Polyhydroxyalkanoates,”Mol Breeding.1, 105-122, 1995.
[6] S. Y. Lee, “Plastic bacteria- progress and prospects for
polyhydroxyalkonate production in bacteria,” Trends Biotechnol.
14,431-438, 1996.
[7] L. LMadison, G. W. Huisman, “Metabolic engineering of
poly(3- hydroxyalkanoates): from DNA to plastic,”Microbiol.
Mol. Biol. Rev. 63, 21–53, 1999
[8] Y. B.Kim, R. W. Lenz, “Polyesters from microorganisms,”
Adv. Biochem. Eng. Biotechnol. 71, 51-79, 2001.
[9] C.S. KReddy, Ghai R Rashmi, V. C.
Kalia,“Polyhydroxyalkanoates: an overview,”Bioresour Technol.
87, 137-146, 2003.
[10] A. Steinbüchel, H. Valentin,“Diversity of bacterial
polyhydroxyalkonicacids,” FEMS microbial Lett. 128, 219-228,
1995.
[11] P. J. Senior, G. A.Beech, G A. F Ritchie, E. A Dawes, “The
role of oxygen limitation of polyhydroxybutyrate during batch
and continuous culture of Azotobacterbeijernckii,”Biochem. J.
128, 1193-1201, 1972.
[12]P. J.Senior, E, A. Dawes, “The regulation of
polyhydroxybutyrate metabolism in
Azotobacterbeijernikii,”Biochem. J. 134, 225- 238, 1973.
[13] D. Byrom,“Polyhydroxyalkonates, Plastics from Microbes:
microbial synthesis of polymers and polymer precursors,”Hanser
Munich. 5 – 33, 1994.
[14] T.Rewate, S. Mavinkurve, “A Rapid hypochlorate method
for extraction of polyhydroxyalkonates from bacterial cells,” Ind.
J. Exp. Biol. 40, 924- 929, 2002.
[15] N. Aarthi, K. V. Ramana, “Identification and
characterization of polyhydroxybutyrate producing Bacillus
cereus and Bacillus mycoides strains,” Int. J. Environ. Sci. 1 (5),
744-756, 2011.
[16] H.Stam, H. W. Verseveld, W. D. E. Van Vries, A. H.
Stouthamer, “Utilization of poly-beetahydroxybutyrate in free
living cultures of RhizobiumORS571,” FEMS MicrobiolLett. 35,
215 – 220, 1986.
[17] R.Tombolini, M. D. Nuti, “Poly (beeta- hydroxyalkonates)
biosynthesis and accumulation by different species,” FEMS
Microbiol. 60, 299 – 304, 1989.
[18] S.Encarnacion, M. Dunn, K. Willams, J. Mora,
“Fermentative and aerobic metabolism in Rhizobium etli,”
J.Bacteriol. 177, 3058 – 3066, 1995.
[19] T. G.Benoit, G. R.Wilson, C. L. Baygh, “Fermentation
during growth and sporulation of Bacillus thuringiensis HD-1,”
LettAppl. Microbiol. 10, 15-18, 1990.
[20] D. H.Nam, D. D. Y Ryu, “Relationship between butirosin
biosynthesis and sporulation in Bacillus
circulans,”AntimicrobAgents Chemother. 2, 789-801, 1985.
[21] A. Santhanam, S. Sasidharan, “Microbial production of
polyhydroxyalkanotes (PHA) fromAlcaligenssp and
Pseudomonas oleovoransusing different carbon sources,” Afr. J.
Biotechnol. 9, 3144 -3150, 2010.
[22] M.Ramachander, S. Girisham, S. M. Reddy,“Production of
PHB (polyhydroxybutyrate) by
RhodopseudomonaspalustrisKU003 under nitrogen limitation,”
Int. J. Appl. Biol. Pharm. Technol. 1, 676 -678, 2010.
[23] K.Sangkharak K, Prasertsan P,“Nutrient optimization for
production of polyhydroxybutyrate from halotolerant
photosynthetic bacteria cultivated under aerobic-dark condition,”
E. J. Biotechnol.11,3-8, 2008.
[24] Nisha V Ramadas, Sudeer Kumar Singh, Carlos Riccardo
Soccol, Ashok Pandey, “Polyhydroxybutyrate production using
agro-industrial residues as substrate by Bacillus sphaericus
NCIM 5149,” Braz. Arch. Biol. Technol. 52, 17-23, 2009.
[25] Thirumala M Sulthanapuram, Vishnuvardha Reddy, S. K.
Mahamood,“Production and characterization of two novel strains
of Bacillussp isolated from soil and activated sludge,” J. Ind.
Microbiol. Biotechnol. 37, 271-278, 2010.
[26] Q.Wu, H. Huang, G. Hu, J. Chen, K. P. Ho, G. Q. Chen,
“Production of poly-3-hydroxybutyrate by Bacillus sp. JMa5
cultivated in molasses media,”Antonie van Leeuwenhoek.
80,111-118, 2001.
[27] T. R.Shamala, A. Chandrashekar, S. V. Vijayendra, L.
Sharma,“Identification of polyhydroxyalkonate (PHA) producing
Bacillus sp. using the polymerase chain reaction,” J. Appl.
Microbiol. 94, 369-374, 2003.
Keywords
Bacteria, growth, PHB production.